Regulatory

Part:BBa_K3978042:Design

Designed by: Minqi Hu   Group: iGEM21_HiZJU-China   (2021-10-21)


neget-ERE-CYC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 29
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 29
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 29
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The estrogen responsive element (ERE) which mediates ERdependent trans-activation is a 13 base pair (bp) palindromic sequence containing 5 bp arms and a 3 bp spacer region, which is the design base of this promoter. The underlined part is the core element of the CYC promoter.


Source

This part was found in this paper: D. Metzger, J. H. White, P. Chambon. The human estrogen receptor functions in yeast [J].Nature, 1988, 334(6177): 31-6.

References